Phenotypic Analysis of Multi-Drug Resistant Cystic Fibrosis Clinical Isolates of Pseudomonas aeruginosa strains

Abstract

Pseudomonas aeruginosa is a Gram negative opportunistic pathogen and a leading cause of lung infection in cystic fibrosis
(CF) patients. This study was focused on characterizing two multi drug resistant (MDR) cystic fibrosis clinical isolates of P.
aeruginosa. These clinical isolates were taken from patients in the Sick Children’s Hospital, Ontario. Genomic analysis and
phenotypic assays were done to assess the multi-drug resistant and virulence phenotype between these isolates compared to wild
type PA01. The strains exhibit very similar resistance profiles apart from meropenem, however a difference is observed in
biofilm formation, virulence, and growth in minimal media.